Oxiconazole Nitrate API Manufacturer in India

Swapnroop Pharma · WHO-GMP Certified · +91 87670 62101

Oxiconazole Nitrate is a broad-spectrum imidazole antifungal API used in pharmaceutical formulations for the topical treatment of superficial fungal skin infections. It belongs to the imidazole class of antifungal agents and acts primarily by interfering with fungal ergosterol biosynthesis.

Oxiconazole nitrate is the nitrate salt of oxiconazole and has the molecular formula C18H13Cl4N3O·HNO3 with a molecular weight of 492.14 g/mol. Its CAS Registry Number is 64211-46-7 and its FDA GSRS/USP identity is associated with UNII RQ8UL4C17S. USP defines Oxiconazole Nitrate as containing 98.0% to 102.0% of oxiconazole nitrate, calculated on the dried basis.

Oxiconazole nitrate is described as a nearly white crystalline powder. It is soluble in methanol, sparingly soluble in ethanol, chloroform and acetone, and very slightly soluble in water.

Chemical Information

  • Product Name: Oxiconazole Nitrate
  • Active Moiety: Oxiconazole
  • CAS Number: 64211-46-7
  • UNII: RQ8UL4C17S
  • Molecular Formula: C18H13Cl4N3O·HNO3
  • Molecular Weight: 492.14 g/mol
  • Oxiconazole Molecular Weight: 429.13 g/mol
  • Chemical Class: Imidazole antifungal
  • Therapeutic Class: Antifungal API
  • Dosage Form Application: Topical pharmaceutical formulations
  • Physical Form: Crystalline powder
  • Appearance: Nearly white crystalline powder
  • Stereochemistry: Z configuration; no chiral center
  • Salt Form: Nitrate
  • Storage: Tight container, room temperature

The USP monograph gives the molecular formula, molecular weight and chemical name, while current FDA labeling confirms the physical description and solubility characteristics.

Key Features

  • Pharmaceutical-grade Oxiconazole Nitrate API
  • Broad-spectrum imidazole antifungal
  • CAS 64211-46-7
  • Molecular weight 492.14 g/mol
  • USP monograph available
  • USP assay specification 98.0–102.0%
  • USP residue on ignition NMT 0.2%
  • USP loss on drying NMT 1.0%
  • USP pH specification 3.0–4.0 for a 10 mg/mL aqueous suspension
  • USP total impurities NMT 0.5%
  • USP individual unspecified impurity NMT 0.10%
  • Five specified oxiconazole-related compounds are individually controlled at NMT 0.15%
  • Nearly white crystalline powder
  • Very slightly soluble in water
  • Soluble in methanol
  • Suitable for topical antifungal pharmaceutical formulations

The numerical values above are taken from the USP 2025 monograph reproduction and official USP preview.

Mechanism of Action

Oxiconazole nitrate exerts its antifungal effect primarily by inhibiting ergosterol biosynthesis in fungal cells.

  • Oxiconazole belongs to the imidazole class of antifungal agents.
  • It interferes with fungal sterol biosynthesis.
  • Ergosterol is an essential component of the fungal cell membrane.
  • Reduction of ergosterol disrupts normal fungal membrane structure and function.
  • The resulting membrane instability inhibits fungal growth and survival.
  • Oxiconazole demonstrates activity against a broad range of pathogenic fungi and dermatophytes.

FDA labeling describes oxiconazole nitrate as an imidazole derivative whose antifungal activity is primarily associated with inhibition of ergosterol biosynthesis.

Pharmaceutical Applications

Oxiconazole Nitrate API is primarily used for development and manufacture of topical antifungal pharmaceutical products.

Its pharmaceutical applications include:

  • Topical antifungal creams
  • Dermatological antifungal formulations
  • Treatment formulations for dermatophyte infections
  • Pharmaceutical development of imidazole antifungal products
  • Analytical reference-standard development
  • Related-substance analysis
  • Stability-indicating analytical studies
  • Topical dosage-form development

Current FDA labeling identifies oxiconazole nitrate cream as a topical dermatologic product.

Antifungal Activity

Oxiconazole has demonstrated in-vitro activity against several clinically relevant fungal organisms.

Its activity includes organisms associated with:

  • Tinea corporis
  • Tinea cruris
  • Tinea pedis
  • Cutaneous candidiasis
  • Other superficial fungal skin infections

Oxiconazole nitrate is incorporated into topical dosage forms because its pharmacological activity is intended primarily at the skin and superficial tissues rather than as a systemic antifungal treatment.

Physicochemical Properties

Oxiconazole nitrate is a highly chlorinated aromatic imidazole compound with low aqueous solubility.

  • Appearance: Nearly white crystalline powder
  • Molecular Weight: 492.14 g/mol
  • Water Solubility: Very slightly soluble
  • Methanol: Soluble
  • Ethanol: Sparingly soluble
  • Chloroform: Sparingly soluble
  • Acetone: Sparingly soluble
  • Physical State: Crystalline solid
  • pH: USP specification 3.0–4.0 for 10 mg/mL aqueous suspension
  • Melting Behavior: Approximately 159–163°C reported in chemical-reference data

FDA labeling confirms the solubility profile of oxiconazole nitrate.

Analytical Characterization

Oxiconazole nitrate has a well-defined USP analytical profile.

Identification

USP identification includes:

  • Infrared spectroscopy
  • HPLC retention-time comparison

The infrared spectrum must conform to the appropriate reference standard, while the oxiconazole peak in the sample must correspond to the standard solution used for the assay.

Assay by HPLC

The current USP method uses liquid chromatography.

Key chromatographic parameters include:

  • Detection wavelength: 220 nm
  • Column: 4.6 mm × 15 cm
  • Particle size: 3 µm
  • Stationary phase: L1
  • Column temperature: 40°C
  • Flow rate: 1.2 mL/min
  • Injection volume: 10 µL
  • Tailing factor: NMT 2.0
  • RSD: NMT 0.73%
  • Assay: 98.0–102.0% on dried basis

These are USP monograph parameters and are therefore considerably stronger than generic commercial specifications.

Organic Impurity Profile

The USP monograph provides specific limits for five related compounds:

  • Oxiconazole Related Compound A — NMT 0.15%
  • Oxiconazole Related Compound B — NMT 0.15%
  • Oxiconazole Related Compound C — NMT 0.15%
  • Oxiconazole Related Compound D — NMT 0.15%
  • Oxiconazole Related Compound E — NMT 0.15%
  • Any individual unspecified impurity — NMT 0.10%
  • Total impurities — NMT 0.50%
  • Reporting threshold — 0.05%

The USP monograph identifies the related compounds chemically, including the E-isomer, oxime-related compound, hydrochloride-related compound, dichlorophenylmethanol and dichlorobenzyl chloride.

Residue on Ignition

The USP specification for residue on ignition is:

NMT 0.2%

This test controls the amount of inorganic residue remaining after ignition of the API.

Loss on Drying

The USP monograph specifies:

NMT 1.0%

The test is performed by drying the sample at 105°C for 4 hours.

pH

The USP monograph specifies pH using a 10 mg/mL suspension in water, rather than a 1% solution.

The acceptance range is:

pH 3.0–4.0

This distinction is important for your CMS because the standard CMS field says “pH (1% Solution),” whereas the actual USP procedure uses a 10 mg/mL suspension.

Solid-State Characteristics

Oxiconazole nitrate is a crystalline material, and the physical form is relevant to pharmaceutical development. FDA's current product information describes it as a nearly white crystalline powder. FDA's topical-product guidance also identifies particle size distribution, crystal habit and polymorphic form as relevant Q3 characteristics for oxiconazole nitrate topical products.

Therefore, pharmaceutical development may appropriately include:

  • XRPD
  • DSC
  • Microscopy
  • Particle-size characterization
  • Crystal-form assessment

Quality and Regulatory Information

Oxiconazole Nitrate has a dedicated USP-NF monograph, making USP the preferred source for the principal API specifications.

The current USP specification includes:

  • Identification
  • HPLC assay
  • Residue on ignition
  • Organic impurities
  • Loss on drying
  • pH
  • Packaging and storage requirements

The USP assay requirement is 98.0–102.0% on the dried basis, while the total organic impurity limit is NMT 0.5%.

Detailed Specifications

Parameter Specification
Appearance white to off-white crystalline powder
Identification IR & HPLC compliant
Assay (HPLC) 98.0% – 102.0%
Loss on Drying NMT 1.0%
Residue on Ignition NMT 0.2%
pH (1% Solution) 3.0 – 4.0
Individual Impurity NMT 0.10%
Total Impurities NMT 0.50%
Melting Point Approximately 159–163°C
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