Olsalazine API Manufacturer in India | Olsalazine Sodium

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Olsalazine is an aminosalicylate anti-inflammatory pharmaceutical compound used primarily in the management of ulcerative colitis. The pharmaceutical form is olsalazine sodium, the disodium salt of olsalazine. It is designed as a colon-targeted prodrug that is converted by intestinal bacterial activity into 5-aminosalicylic acid (mesalamine, 5-ASA), the pharmacologically active anti-inflammatory moiety.

Olsalazine sodium is chemically known as disodium 3,3′-diazenediylbis(6-hydroxybenzoate). The compound contains an azo linkage connecting two salicylate-derived moieties. Current BP/Ph. Eur. 2025 information describes it as a yellow, fine, crystalline powder with a content requirement of 98.0% to 102.0% on the dried substance basis.

Chemical Information

  • API Name: Olsalazine Sodium
  • Active Moiety: Olsalazine
  • CAS Number: 6054-98-4
  • Parent Olsalazine CAS: 15722-48-2
  • Molecular Formula: C14H8N2Na2O6
  • Molecular Weight: 346.21 g/mol
  • Chemical Class: Aminosalicylate / azo compound
  • IUPAC Description: Disodium 3,3′-diazenediylbis(6-hydroxybenzoate)
  • Stereochemistry: Achiral
  • Physical Form: Yellow, fine crystalline powder
  • Pharmaceutical Form: Disodium salt
  • Pharmacological Role: Colon-targeted prodrug of mesalamine
  • Compendial Reference: BP 2025 / Ph. Eur. monograph 1457

The identity and chemical information are supported by the BP/Ph. Eur. monograph, PubChem and official product labeling.

Mechanism of Action

Olsalazine sodium acts primarily as a prodrug of mesalamine (5-aminosalicylic acid).

  • Olsalazine reaches the colon largely in an intact form.
  • Colonic bacterial enzymes cleave the azo linkage.
  • This produces two molecules of 5-aminosalicylic acid.
  • Mesalamine acts locally on the colonic mucosa.
  • The resulting anti-inflammatory activity helps reduce inflammation associated with ulcerative colitis.
  • The azo-linked structure therefore provides a mechanism for preferential delivery of the active 5-ASA moiety to the colon.

The drug is classified as an aminosalicylate and is used for the treatment of ulcerative colitis.

Key Features

  • High-purity pharmaceutical-grade olsalazine sodium
  • Compendial content specification of 98.0–102.0% on a dried basis
  • Defined BP/Ph. Eur. related-substances methodology
  • Individual specified impurities A–I controlled by LC
  • Total related substances controlled to NMT 2.0%
  • Loss on drying controlled to NMT 2.0%
  • Acetate controlled to NMT 1.0%
  • Methanesulfonic acid controlled to NMT 0.3%
  • UV identification includes absorption maxima at 255 nm and 362 nm
  • UV absorbance ratio A255/A362 = 0.53–0.56
  • Yellow, fine crystalline powder
  • Demonstrates polymorphism and therefore solid-state characterization can be important for pharmaceutical development
  • Suitable for analytical characterization by UV, IR, TLC and liquid chromatography

These values correspond to the BP 2025 / Ph. Eur. 11.6 monograph.

Pharmaceutical Applications

Olsalazine sodium is primarily associated with:

  • Treatment of ulcerative colitis
  • Aminosalicylate-based gastrointestinal therapy
  • Colon-targeted delivery of mesalamine
  • Pharmaceutical development of oral anti-inflammatory formulations
  • Analytical and formulation research involving 5-ASA prodrugs

Olsalazine is converted into mesalamine by bacterial metabolism in the colon, allowing the active anti-inflammatory component to act locally in the gastrointestinal tract.

Physicochemical Characteristics

Olsalazine sodium is reported as a yellow, fine, crystalline powder in the BP/Ph. Eur. monograph.

  • Appearance: Yellow, fine, crystalline powder
  • Water Solubility: Sparingly soluble according to BP/Ph. Eur.
  • DMSO Solubility: Soluble
  • Methanol Solubility: Very slightly soluble
  • Polymorphism: Present
  • Melting Behavior: Melts with decomposition at approximately 240°C according to product labeling
  • Molecular Weight: 346.21 g/mol

The BP/Ph. Eur. characterization describes it as sparingly soluble in water, soluble in dimethyl sulfoxide and very slightly soluble in methanol. The US product labeling reports decomposition at approximately 240°C and describes the material as soluble in water and DMSO; for a CMS API specification, the current compendial description is preferred.

Analytical Characterization

Identification

BP/Ph. Eur. provides multiple identification approaches:

  • UV-Visible spectrophotometry
  • Infrared absorption spectrophotometry
  • Thin-layer chromatography
  • Sodium identification reaction

For UV identification, absorption maxima occur at 255 nm and 362 nm, with an A255/A362 ratio of 0.53–0.56.

Related Substances

The BP/Ph. Eur. monograph identifies nine specified impurities:

  • Impurity A
  • Impurity B
  • Impurity C
  • Impurity D
  • Impurity E
  • Impurity F
  • Impurity G
  • Impurity H
  • Impurity I

Each specified impurity has a principal limit of NMT 1.0%, with the additional requirement that no more than one impurity peak may exceed 0.5%. The total related substances limit is NMT 2.0%, with a disregard limit of 0.025%.

Process-Related Impurities

The current monograph separately controls:

  • Acetate: NMT 1.0%
  • Methanesulfonic acid: NMT 0.3%

This is particularly relevant when developing or qualifying olsalazine sodium API manufacturing processes.

Polymorphism

Olsalazine sodium shows polymorphism according to the BP/Ph. Eur. monograph. Therefore, solid-state characterization such as XRPD can be relevant when establishing the physical form of API batches and maintaining consistency during pharmaceutical development.

Quality and Compliance

Quality evaluation of olsalazine sodium can include:

  • Compendial identity testing
  • Assay/content determination
  • Related-substances profiling
  • Acetate determination
  • Methanesulfonic acid determination
  • Loss on drying
  • Appearance evaluation
  • UV-Visible characterization
  • IR identification
  • TLC identification
  • Solid-state/polymorphic characterization

The BP 2025 / Ph. Eur. monograph provides numerical acceptance criteria for content, related substances, acetate, methanesulfonic acid and loss on drying.

Storage

Olsalazine sodium should be stored in a tightly closed, appropriately controlled pharmaceutical container, protected from excessive moisture and unsuitable environmental conditions. Actual storage conditions should follow the validated API stability data and approved manufacturer specification.

Detailed Specifications

Parameter Specification
Appearance White crystalline powder or crystals
Identification IR & HPLC compliant
Assay (HPLC) 98.0% – 102.0%
Loss on Drying NMT 2.0%
Residue on Ignition NMT 0.5%
Individual Impurity NMT 0.5%
Total Impurities NMT 2.0%
Water Content NMT 2.0%
Melting Point Approximately 240°C with decomposition
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