Nordihydroguaiaretic Acid API Manufacturer in India

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Nordihydroguaiaretic Acid API

Nordihydroguaiaretic Acid, commonly abbreviated as NDGA, is a naturally occurring lignan compound containing four phenolic hydroxyl groups. It is associated with antioxidant activity and inhibition of lipoxygenase enzymes and occurs naturally in plants including Larrea tridentata (creosote bush). PubChem identifies Nordihydroguaiaretic Acid as a lignan, catechol-containing compound and antioxidant.

Nordihydroguaiaretic Acid has applications in pharmaceutical research, biochemical research, antioxidant studies and lipoxygenase-related research.

Nordihydroguaiaretic Acid API Manufacturer in India

Nordihydroguaiaretic Acid is a specialized pharmaceutical and research-use chemical ingredient used in the development and analytical investigation of antioxidant and enzyme-inhibition systems.

Important quality attributes include identity, assay/purity, related substances, water content, residue on ignition, elemental impurities, melting point and solubility.

Chemical Information

CAS Number: 500-38-9

Molecular Formula: C₁₈H₂₂O₄

Molecular Weight: 302.36 g/mol

Chemical Name: 4,4'-(2,3-Dimethyltetramethylene)-dipyrocatechol

IUPAC Name: 4-[4-(3,4-dihydroxyphenyl)-2,3-dimethylbutyl]benzene-1,2-diol

Synonyms: NDGA, Nordihydroguaiaretic Acid, Dihydronorguaiaretic Acid, Masoprocol

Drug/Class Type: Natural Product / Lignan

Therapeutic Area: Pharmaceutical and Biochemical Research

Molecule Type: Natural Product / Small Molecule

Biological Source: Larrea tridentata / Larrea divaricata

PubChem confirms CAS 500-38-9, formula C₁₈H₂₂O₄ and molecular weight 302.4 g/mol. JECFA reports a formula weight of 302.36.

Mechanism of Action

Nordihydroguaiaretic Acid is recognized particularly for its ability to inhibit lipoxygenase enzymes, which participate in the metabolism of arachidonic acid and formation of lipid mediators.

Its biological activities include:

  • Lipoxygenase inhibition
  • Antioxidant activity
  • Modulation of lipid oxidation
  • Research activity involving oxidative stress
  • Investigation of cellular signaling pathways
  • Investigation of ferroptosis-related mechanisms

PubChem describes Nordihydroguaiaretic Acid as a potent lipoxygenase inhibitor and antioxidant.

Pharmaceutical and Research Applications

Nordihydroguaiaretic Acid is relevant to:

  • Antioxidant research
  • Lipoxygenase inhibition studies
  • Pharmaceutical research
  • Biochemical research
  • Natural-product research
  • Oxidative-stress research
  • Drug-discovery research
  • Enzyme-inhibition studies
  • Cell biology research
  • Analytical research

Antioxidant Applications

NDGA contains multiple phenolic hydroxyl groups that contribute to its antioxidant properties. It has been investigated for its ability to interact with oxidative processes and free-radical pathways.

JECFA identifies Nordihydroguaiaretic Acid as an antioxidant and describes it as a white to greyish-white crystalline solid.

Lipoxygenase Research

Lipoxygenases are enzymes involved in the oxidation of polyunsaturated fatty acids.

Nordihydroguaiaretic Acid has historically been studied as a lipoxygenase inhibitor, making it useful as a research compound for investigating lipid oxidation, inflammatory signaling and related biochemical pathways.

Physical Characteristics

Nordihydroguaiaretic Acid is generally described as a white to greyish-white crystalline solid or white/off-white crystalline powder.

JECFA reports a melting point of approximately 184°C, while commercial analytical specifications report ranges around 184–189°C.

Solubility

Nordihydroguaiaretic Acid is insoluble or only slightly soluble in water. It is soluble in organic solvents including ethanol and ether. JECFA specifically describes it as freely soluble in ethanol and ether, and in propylene glycol at elevated temperature.

Natural Source

Nordihydroguaiaretic Acid is naturally associated with the creosote bush, Larrea tridentata, and related Larrea species.

It is also reported as a natural product in several plant species.

Manufacturing Process

A controlled Nordihydroguaiaretic Acid manufacturing process may involve:

  1. Qualification of starting materials
  2. Selection and control of appropriate natural or synthetic starting material
  3. Extraction or controlled chemical synthesis
  4. Filtration
  5. Concentration
  6. Purification
  7. Crystallization
  8. Washing
  9. Controlled drying
  10. Milling, where required
  11. Particle-size control
  12. Final analytical testing
  13. GMP-compliant packaging

Quality Control Testing

Typical Nordihydroguaiaretic Acid quality-control testing may include:

  • Identification by IR
  • HPLC identification
  • Assay by HPLC
  • Related substances
  • Water content
  • Loss on drying
  • Residue on ignition
  • Elemental impurities
  • Heavy metals
  • Melting point
  • Residual solvents
  • Stability testing

Storage

Store Nordihydroguaiaretic Acid in a tightly closed, light-resistant container under controlled conditions, protected from excessive heat, moisture and incompatible oxidizing agents.

Some commercial analytical products recommend refrigerated storage, while storage requirements for a pharmaceutical-grade material should follow its validated stability data and approved specification.

Pharmaceutical Research

Nordihydroguaiaretic Acid is relevant to research involving:

  • Lipoxygenase inhibition
  • Antioxidant mechanisms
  • Oxidative stress
  • Lipid oxidation
  • Natural products
  • Lignan chemistry
  • Ferroptosis research
  • Cellular signaling
  • Enzyme inhibition
  • Drug discovery
  • Pharmaceutical analytical research

Nordihydroguaiaretic Acid API Summary

Nordihydroguaiaretic Acid is a natural lignan and antioxidant compound with CAS 500-38-9, molecular formula C₁₈H₂₂O₄ and molecular weight 302.36 g/mol. It is associated particularly with lipoxygenase inhibition and antioxidant research. JECFA reports an assay range of 95–102%, melting point of approximately 184°C, and describes the material as a white to greyish-white crystalline solid.

Detailed Specifications

Parameter Specification
Appearance White crystalline powder or crystals
Identification IR & HPLC compliant
Assay (HPLC) 95.0% – 102.0%
Loss on Drying NMT 2.0%
Residue on Ignition NMT 0.5%
Individual Impurity NMT 0.10%
Total Impurities NMT 0.50%
Water Content NMT 1.0%
Melting Point About 184°C
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