Nilutamide API Manufacturer in India

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Nilutamide API

Nilutamide is a synthetic non-steroidal antiandrogen active pharmaceutical ingredient (API) belonging to the androgen-receptor antagonist class. It is used in androgen-deprivation therapy and is primarily associated with the treatment of metastatic prostate cancer. Nilutamide works by blocking the action of androgens at the androgen receptor.

Nilutamide API Manufacturer in India

Nilutamide is a specialized oncology pharmaceutical API used in the development and manufacture of antiandrogen formulations and in pharmaceutical research.

The European Pharmacopoeia monograph identifies Nilutamide as 5,5-dimethyl-3-[4-nitro-3-(trifluoromethyl)phenyl]imidazolidine-2,4-dione and specifies a content of 98.0%–102.0% on the anhydrous substance.

Chemical Information

CAS Number: 63612-50-0

Molecular Formula: C₁₂H₁₀F₃N₃O₄

Molecular Weight: 317.22 g/mol

Chemical Name: 5,5-Dimethyl-3-[4-nitro-3-(trifluoromethyl)phenyl]imidazolidine-2,4-dione

IUPAC Name: 5,5-Dimethyl-3-[4-nitro-3-(trifluoromethyl)phenyl]imidazolidine-2,4-dione

Drug Class: Non-Steroidal Antiandrogen

Therapeutic Class: Antiandrogen / Antineoplastic Agent

Therapeutic Area: Oncology / Prostate Cancer

Molecule Type: Synthetic Small Molecule

ATC Code: L02BB02

The molecular identity and physicochemical information are supported by IUPHAR/BPS and official/regulatory pharmaceutical sources.

Mechanism of Action

Nilutamide is a non-steroidal androgen receptor antagonist. It competitively interferes with androgen binding to the androgen receptor, reducing androgen-dependent signaling.

Its pharmacological activity includes:

  • Androgen receptor antagonism
  • Competitive inhibition of androgen action
  • Reduction of androgen-receptor signaling
  • Suppression of androgen-dependent cellular activity
  • Inhibition of androgen-stimulated prostate cancer growth

Nilutamide is used as part of androgen-deprivation treatment in prostate cancer.

Pharmaceutical Applications

Nilutamide API is relevant to:

  • Prostate cancer pharmaceutical formulations
  • Antiandrogen drug development
  • Androgen receptor research
  • Oncology drug development
  • Hormonal cancer therapy research
  • Pharmaceutical formulation development
  • API analytical testing
  • Pharmaceutical research and development
  • Cancer pharmacology
  • Androgen signaling research

Oncology Applications

Nilutamide has been used in combination with surgical castration for the treatment of metastatic prostate cancer. Its role is to inhibit androgen action at the receptor level after androgen deprivation.

Androgen Receptor Antagonism

Nilutamide binds to androgen receptors and prevents activation by endogenous androgens. This mechanism distinguishes it from steroidal antiandrogens and makes Nilutamide relevant to research involving androgen receptor signaling and prostate cancer biology.

Physical Characteristics

Nilutamide is described as a white or almost white powder in the European Pharmacopoeia monograph. Regulatory information describes it as a microcrystalline, white to practically white powder.

Nilutamide has a molecular weight of approximately 317.22 g/mol.

Solubility

Regulatory drug-substance information describes Nilutamide as:

  • Slightly soluble in water
  • Freely soluble in ethyl acetate
  • Freely soluble in acetone
  • Freely soluble in chloroform
  • Freely soluble in ethanol
  • Freely soluble in dichloromethane
  • Freely soluble in methanol 

Manufacturing Process

A controlled Nilutamide API manufacturing process may involve:

  1. Qualification of starting materials
  2. Preparation of substituted aromatic intermediates
  3. Introduction of the nitro and trifluoromethyl substituents
  4. Preparation of the imidazolidinedione intermediate
  5. Controlled coupling reaction
  6. Reaction monitoring
  7. Purification
  8. Crystallization
  9. Filtration and washing
  10. Controlled drying
  11. Final analytical testing
  12. GMP-compliant packaging

Quality Control Testing

Typical Nilutamide API quality control may include:

  • Identification by IR
  • Assay by liquid chromatography
  • Related substances
  • Water content
  • Sulfated ash
  • Heavy metals / elemental impurities
  • Residual solvents
  • Stability testing

The European Pharmacopoeia monograph specifically establishes tests for related substances, water, sulfated ash and assay.

Storage

Store Nilutamide API protected from light in a tightly closed container under appropriate pharmaceutical storage conditions. The European Pharmacopoeia monograph specifically states protected from light.

Pharmaceutical Research

Nilutamide is relevant to research involving:

  • Androgen receptor antagonists
  • Prostate cancer
  • Androgen signaling
  • Antiandrogen drug development
  • Hormone-dependent cancers
  • Oncology pharmacology
  • Cancer drug discovery
  • Androgen deprivation therapy
  • Pharmaceutical formulation development
  • API analytical research

 

Detailed Specifications

Parameter Specification
Appearance White crystalline powder or crystals
Identification IR & HPLC compliant
Assay (HPLC) 98.0% – 102.0%
Loss on Drying NMT 0.5%
Residue on Ignition NMT 0.1%
Heavy Metals NMT 20 ppm
Individual Impurity NMT 0.10% each
Total Impurities NMT 0.30%
Water Content NMT 0.5%
Melting Point 153–156°C
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