Netilmicin Sulfate API Manufacturer in India

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Netilmicin Sulfate API

Netilmicin Sulfate is a semi-synthetic aminoglycoside antibacterial active pharmaceutical ingredient (API) derived from sisomicin. It belongs to the aminoglycoside class of antibacterial agents and is active against susceptible Gram-negative bacteria.

Netilmicin Sulfate is used in pharmaceutical development and manufacture of antibacterial formulations and is particularly associated with treatment of serious infections caused by susceptible aerobic Gram-negative organisms.

Netilmicin Sulfate API Manufacturer in India

Netilmicin Sulfate is a specialized aminoglycoside antibacterial API used in pharmaceutical formulation development, antimicrobial research, analytical testing and pharmaceutical manufacturing.

The USP monograph defines Netilmicin Sulfate as the sulfate salt and specifies potency based on the netilmicin active moiety. USP states that it has a potency equivalent to not less than 595 µg of netilmicin per mg, calculated on the dried basis.

Chemical Information

CAS Number: 56391-57-2

Molecular Formula: (C₂₁H₄₁N₅O₇)₂·5H₂SO₄

Molecular Weight: 1441.55 g/mol

Active Moiety Formula: C₂₁H₄₁N₅O₇

Active Moiety Molecular Weight: 475.6 g/mol

Chemical Name: Netilmicin sulfate (2:5)

Drug Class: Aminoglycoside Antibacterial

Therapeutic Class: Aminoglycoside Antibiotic

Therapeutic Area: Infectious Diseases

Molecule Type: Semi-synthetic Aminoglycoside

Development Code / Synonym: Netromycin / SCH 20569

USP identifies the sulfate salt with CAS 56391-57-2 and molecular weight 1441.55 g/mol, while PubChem gives the free netilmicin active moiety as CAS 56391-56-1, formula C₂₁H₄₁N₅O₇, and molecular weight 475.6 g/mol.

Mechanism of Action

Netilmicin is an aminoglycoside that exerts antibacterial activity primarily through interaction with bacterial ribosomes.

Its antibacterial mechanism includes:

  • Binding to the bacterial 30S ribosomal subunit
  • Interference with bacterial protein synthesis
  • Misreading of messenger RNA
  • Production of abnormal bacterial proteins
  • Inhibition of bacterial growth
  • Bactericidal activity against susceptible organisms

Pharmaceutical Applications

Netilmicin Sulfate API is relevant to:

  • Antibacterial formulation development
  • Aminoglycoside antibiotic formulations
  • Injectable pharmaceutical preparations
  • Antimicrobial research
  • Gram-negative bacterial infection research
  • Pharmaceutical API manufacturing
  • Microbiological research
  • Antibiotic drug development
  • Analytical quality control
  • Pharmaceutical formulation research

Antibacterial Applications

Netilmicin is associated primarily with antibacterial activity against susceptible aerobic Gram-negative bacteria. Its use is determined by bacterial susceptibility, clinical indication and applicable regulatory requirements.

Netilmicin Sulfate is available as a pharmaceutical active ingredient for development of formulations such as sterile preparations.

Physical Characteristics

Netilmicin Sulfate is described in the Ph. Eur. material as a white or yellowish-white powder and is very hygroscopic. It is very soluble in water and practically insoluble in acetone and alcohol.

Manufacturing Process

A controlled Netilmicin Sulfate API manufacturing process may involve:

  1. Qualification of sisomicin starting material
  2. Controlled semi-synthetic modification
  3. Introduction of the required N-ethyl functionality
  4. Reaction monitoring
  5. Purification
  6. Conversion to the sulfate salt
  7. Crystallization
  8. Filtration and washing
  9. Controlled drying
  10. Milling, where required
  11. Potency and chromatographic testing
  12. GMP-compliant packaging

Quality Control Testing

Typical Netilmicin Sulfate API testing may include:

  • Identification by chromatographic methods
  • Sulfate identification
  • Assay/potency
  • Related substances
  • Individual impurities
  • Total impurities
  • pH
  • Loss on drying
  • Residue on ignition
  • Specific optical rotation
  • Sulfate content
  • Microbiological quality, where applicable
  • Stability testing

Storage

Store Netilmicin Sulfate API in a tightly closed container protected from moisture, under controlled pharmaceutical storage conditions.

Because the substance is described as very hygroscopic, moisture protection is particularly important during storage and handling.

Detailed Specifications

Parameter Specification
Appearance White crystalline powder or crystals
Identification IR & HPLC compliant
Assay (HPLC) 98.0% – 102.0%
Loss on Drying NMT 15.0%
Residue on Ignition NMT 1.0%
Individual Impurity NMT 1.0%
Total Impurities NMT 5.0%
Water Content NMT 15.0%
Specific Rotation +88.0° to +96.0°
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