Nefazodone Hydrochloride API Manufacturer in India

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Nefazodone Hydrochloride API

Nefazodone Hydrochloride is a synthetic antidepressant active pharmaceutical ingredient (API) belonging to the class of serotonin modulators. It is a hydrochloride salt of nefazodone and has pharmacological activity involving serotonin receptors and serotonin reuptake.

Nefazodone is structurally related to the phenylpiperazine class of compounds and has a pharmacological profile that differs from conventional selective serotonin reuptake inhibitors (SSRIs). It acts primarily as a 5-HT2A receptor antagonist while also inhibiting serotonin reuptake.

Nefazodone Hydrochloride is used as a pharmaceutical active ingredient in the development and manufacture of oral antidepressant formulations.

Nefazodone Hydrochloride API Manufacturer in India

Nefazodone Hydrochloride is a well-characterized small-molecule pharmaceutical API used in pharmaceutical formulation development, analytical research and quality-control applications.

The USP-NF contains a dedicated Nefazodone Hydrochloride monograph, confirming established compendial analytical requirements for the substance.

Important quality attributes include:

  • Identity
  • Assay
  • Related substances
  • Water/loss on drying
  • Residue on ignition
  • Residual solvents
  • Elemental impurities
  • Chromatographic purity
  • Stability

Chemical Information

Product Name: Nefazodone Hydrochloride

CAS Number: 82752-99-6

Molecular Formula: C25H32ClN5O2 · HCl

Molecular Weight: 506.47 g/mol

Drug Class: Serotonin Modulator / Antidepressant

Therapeutic Class: Antidepressant

Therapeutic Area: Psychiatry / Central Nervous System

Molecule Type: Synthetic Small Molecule

The USP identifies Nefazodone Hydrochloride with the molecular formula C25H32ClN5O2·HCl and molecular weight 506.47 g/mol.

Mechanism of Action

Nefazodone has a distinctive serotonergic mechanism involving both serotonin receptors and serotonin transport.

Its pharmacological actions include:

  • Antagonism of 5-HT2A serotonin receptors
  • Inhibition of serotonin reuptake
  • Modulation of serotonergic neurotransmission
  • Additional activity at other serotonin receptor subtypes
  • Limited affinity for muscarinic cholinergic receptors compared with some older antidepressants

Nefazodone's mixed mechanism differentiates it from antidepressants that primarily inhibit serotonin reuptake.

Pharmaceutical Applications

Nefazodone Hydrochloride API is relevant to:

  • Antidepressant formulation development
  • Serotonin-modulating drug research
  • CNS pharmaceutical development
  • Psychiatric drug research
  • Pharmaceutical formulation development
  • Analytical method development
  • Quality-control testing
  • Stability studies
  • Pharmaceutical research and development

Therapeutic Application

Nefazodone has been used as an antidepressant for the treatment of major depressive disorder.

Its regulatory status and availability vary by country. In the United States, nefazodone-containing products have had important safety considerations, particularly concerning severe liver injury, and its clinical use has consequently been restricted. Product-specific regulatory information should therefore be followed for the applicable market.

Physical Characteristics

Nefazodone Hydrochloride is a pharmaceutical-grade crystalline solid. The API's physical characteristics, including particle size, crystallinity and moisture characteristics, can influence pharmaceutical formulation performance.

The final physical specification should be controlled according to the applicable compendial monograph or approved API specification.

Manufacturing Process

A controlled Nefazodone Hydrochloride manufacturing process may involve:

  • Qualification of starting materials
  • Preparation of substituted piperazine intermediates
  • Controlled chemical synthesis
  • Formation of the triazolone-containing structure
  • Reaction monitoring
  • Purification
  • Conversion to hydrochloride salt
  • Crystallization
  • Filtration
  • Washing
  • Controlled drying
  • Milling, where required
  • Final analytical testing
  • GMP-compliant packaging

The exact commercial manufacturing route should be based on the manufacturer's validated process and regulatory documentation.

Quality Control Testing

Nefazodone Hydrochloride API quality control may include:

  • Identification by IR
  • Chromatographic identification
  • Assay by HPLC or validated compendial procedure
  • Organic impurities
  • Specified related compounds
  • Unspecified impurities
  • Total impurities
  • Loss on drying
  • Residue on ignition
  • Residual solvents
  • Elemental impurities
  • Stability testing

The USP monograph specifically provides analytical controls for assay and organic impurities.

USP Assay Requirement

The USP monograph specifies that Nefazodone Hydrochloride contains not less than 98.0% and not more than 102.0% of C25H32ClN5O2·HCl, calculated on the dried basis.

This makes 98.0%–102.0% a significantly stronger CMS value than a generic assumed API assay range.

Organic Impurities

The USP monograph identifies Nefazodone Related Compound A and Nefazodone Related Compound B as specified impurities.

The older official USP monograph gives the following acceptance criteria:

  • Nefazodone Related Compound A: NMT 0.2%
  • Nefazodone Related Compound B: NMT 0.2%
  • Any unknown impurity: NMT 0.1%
  • Total impurities: NMT 0.5%

The USP has subsequently proposed/revised the organic-impurity presentation and reference-standard arrangements, so the current official USP-NF version should be checked when implementing the final CMS specification.

Loss on Drying

The official USP material specifies loss on drying of not more than 0.5%, using vacuum drying at 105°C for 3 hours.

Residue on Ignition

The USP specification identifies residue on ignition NMT 0.1%.

Heavy Metals

The historical USP monograph specifies heavy metals at 0.001%. For a current commercial API specification, however, elemental impurities should be controlled according to the applicable current compendial requirements and ICH Q3D framework rather than automatically carrying forward an old heavy-metals test.

Solubility

The USP monograph states that a 25 mg/mL solution in methanol meets the applicable completeness-of-solution requirement.

For website CMS purposes, it is safer to describe solubility as soluble in methanol rather than inventing a quantitative water-solubility value.

Storage

The historical USP monograph specifies preservation in tight containers and storage between 15°C and 30°C.

For a commercial product page, storage conditions should ultimately correspond to the current applicable API specification and validated stability data.

Pharmaceutical Research

Nefazodone Hydrochloride is relevant to research involving:

  • Serotonin receptor pharmacology
  • 5-HT2A receptor antagonism
  • Serotonin reuptake inhibition
  • Antidepressant drug development
  • CNS pharmaceutical research
  • Psychiatric drug research
  • Serotonergic signaling
  • Analytical method development
  • Pharmaceutical formulation research

Detailed Specifications

Parameter Specification
Appearance White crystalline powder or crystals
Identification IR & HPLC compliant
Assay (HPLC) 98.0% – 102.0%
Loss on Drying NMT 0.5%
Residue on Ignition NMT 0.1%
Individual Impurity NMT 0.5%
Total Impurities NMT 0.5%
Water Content NMT 0.5%
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