Naxitamab API Manufacturer in India

Swapnroop Pharma · WHO-GMP Certified · +91 87670 62101

Naxitamab API

Naxitamab, also known as naxitamab-gqgk and development code hu3F8, is a humanized monoclonal antibody that targets the disialoganglioside GD2. It is an oncology-focused biological therapeutic used in the treatment of certain high-risk neuroblastoma cases.

Naxitamab is a complex protein-based biological molecule rather than a conventional small-molecule API. Its quality evaluation therefore involves antibody identity, purity, aggregation, charge variants, glycosylation and biological activity.

Naxitamab API Manufacturer in India

Naxitamab is a specialized biopharmaceutical API relevant to antibody-drug development and targeted oncology applications. The material requires appropriate biological manufacturing, purification and analytical characterization.

Biological Information

CAS Number: Not established / not routinely applicable

Molecular Formula: Not applicable as a conventional small molecule

Molecular Weight: Approximately 150 kDa

Molecule Type: Humanized monoclonal antibody

Antibody Isotype: IgG1

Target: GD2 (disialoganglioside)

Drug Class: Monoclonal Antibody

Therapeutic Area: Oncology

Development Code: hu3F8

Mechanism of Action

Naxitamab binds to GD2, a ganglioside expressed at high levels on neuroblastoma cells and certain other tumors.

Its activity involves:

  • Specific binding to GD2
  • Recognition of GD2-expressing tumor cells
  • Antibody-dependent cellular cytotoxicity (ADCC)
  • Complement-dependent cytotoxicity (CDC)
  • Immune-mediated tumor-cell killing

By binding GD2 on tumor cells, naxitamab can recruit immune effector mechanisms that contribute to destruction of targeted malignant cells.

Pharmaceutical Applications

Naxitamab is relevant to:

  • Neuroblastoma treatment development
  • GD2-targeted oncology research
  • Monoclonal antibody development
  • Pediatric oncology research
  • Cancer immunotherapy research
  • Biopharmaceutical development
  • Analytical characterization of therapeutic antibodies

Oncology Applications

Naxitamab is particularly associated with high-risk neuroblastoma. The antibody targets GD2, a cell-surface antigen highly expressed by neuroblastoma cells, making it an important target for antibody-based cancer therapy.

Physical Characteristics

Naxitamab is a protein-based monoclonal antibody. Its physical properties depend on the formulation and manufacturing process. Biological preparations are typically evaluated for appearance, concentration, aggregation, purity and other protein-quality attributes rather than conventional small-molecule characteristics such as melting point.

Manufacturing Process

A controlled monoclonal-antibody manufacturing process may generally include:

  1. Qualified cell-line development
  2. Cell-culture production
  3. Harvest and clarification
  4. Protein capture
  5. Chromatographic purification
  6. Viral clearance
  7. Polishing
  8. Concentration and buffer exchange
  9. Sterile filtration
  10. Formulation
  11. Final analytical testing
  12. Controlled packaging and storage

Quality Control Testing

Typical Naxitamab biological-product characterization may include:

  • Identity testing
  • Protein concentration
  • Purity
  • Size-exclusion chromatography
  • Aggregate determination
  • Charge-variant analysis
  • Peptide mapping
  • Glycosylation characterization
  • Host-cell protein
  • Residual host-cell DNA
  • Endotoxin
  • Bioburden
  • Sterility, where applicable
  • Biological potency
  • Binding activity to GD2

Storage

Store Naxitamab according to the validated storage conditions of the applicable formulation and approved product specification. Temperature control, light protection and freeze-thaw limitations should be established from validated stability data.

Pharmaceutical Research

Naxitamab is relevant to research involving:

  • GD2 biology
  • Neuroblastoma
  • Antibody-dependent cellular cytotoxicity
  • Complement-dependent cytotoxicity
  • Cancer immunotherapy
  • Monoclonal antibody engineering
  • Pediatric oncology
  • Therapeutic antibody development
  • Biological potency assays

Detailed Specifications

Parameter Specification
Appearance White crystalline powder or crystals
Identification IR & HPLC compliant
Assay (HPLC) 98.0% – 102.0%
Loss on Drying NMT 1.0%
Residue on Ignition NMT 0.5%
Individual Impurity NMT 0.5%
Total Impurities ≤ 1.5%
Water Content NMT 1.0%
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