Nafarelin API Manufacturer in India

Swapnroop Pharma · WHO-GMP Certified · +91 87670 62101

Nafarelin API

Nafarelin is a synthetic gonadotropin-releasing hormone (GnRH) agonist and peptide active pharmaceutical ingredient used in pharmaceutical formulations and endocrine research. It is a synthetic analogue of gonadotropin-releasing hormone that produces an initial stimulation of the hypothalamic-pituitary-gonadal axis followed by suppression of gonadotropin secretion with continuous administration.

Nafarelin is commonly used in pharmaceutical development as nafarelin acetate, the acetate salt of the peptide. It is associated with therapeutic applications involving hormone-dependent conditions and reproductive endocrinology.

Nafarelin API Manufacturer in India

Nafarelin is a specialized peptide pharmaceutical API used for the development and manufacture of formulations involving GnRH receptor agonist activity.

Important API quality attributes include identity, peptide purity, assay, related substances, water content, residual solvents, counter-ion content, elemental impurities and microbiological quality, as applicable to the approved specification.

Chemical Information

CAS Number: 76932-56-4

Molecular Formula: C66H83N17O13

Molecular Weight: 1322.48 g/mol

Drug Class: Gonadotropin-Releasing Hormone Agonist

Therapeutic Class: Gonadotropin-Releasing Hormone Analogue

Therapeutic Area: Endocrinology / Reproductive Health

Molecule Type: Synthetic Peptide

Pharmaceutical Form: Nafarelin acetate

Mechanism of Action

Nafarelin acts as a potent agonist of the gonadotropin-releasing hormone receptor (GnRH receptor).

Its pharmacological activity includes:

  • GnRH receptor activation
  • Initial stimulation of LH and FSH secretion
  • Continued stimulation leading to receptor desensitization
  • Suppression of LH and FSH secretion
  • Reduction of gonadal sex-hormone production
  • Modulation of reproductive hormone pathways

With continuous exposure, Nafarelin suppresses pituitary gonadotropin secretion, resulting in reduced ovarian or testicular steroidogenesis.

Pharmaceutical Applications

Nafarelin API is used in pharmaceutical development for:

  • Endometriosis treatment formulations
  • Hormone-dependent reproductive disorders
  • GnRH agonist pharmaceutical development
  • Reproductive endocrinology research
  • Gonadotropin regulation studies
  • Hormone-dependent disease research
  • Pharmaceutical research and development

Endocrinology Applications

Nafarelin is particularly relevant to reproductive endocrinology because prolonged GnRH receptor stimulation suppresses pituitary secretion of luteinizing hormone (LH) and follicle-stimulating hormone (FSH).

This hormonal suppression reduces ovarian estrogen production and is an important pharmacological mechanism in the management of hormone-dependent conditions such as endometriosis.

Physical Characteristics

Nafarelin is a synthetic peptide and is generally handled as a pharmaceutical peptide substance. Its physical characteristics, including appearance, water content and solid-state properties, should be controlled according to the applicable API specification.

Nafarelin is commonly used in its acetate salt form for pharmaceutical applications.

Manufacturing Process

A controlled Nafarelin API manufacturing process may involve:

  1. Selection and qualification of peptide starting materials
  2. Stepwise peptide synthesis
  3. Protection and deprotection of amino-acid residues
  4. Peptide chain assembly
  5. Cleavage from the solid support, where applicable
  6. Crude peptide isolation
  7. Purification using chromatographic techniques
  8. Removal of process-related impurities
  9. Conversion to the required salt form
  10. Concentration and isolation
  11. Controlled drying
  12. Final analytical testing
  13. GMP-compliant packaging

Quality Control Testing

Typical Nafarelin API testing may include:

  • Identification by chromatographic methods
  • Peptide identity by mass spectrometry
  • HPLC assay or peptide content
  • Related peptide substances
  • Degradation products
  • Water content
  • Residual solvents
  • Acetate content, where applicable
  • Elemental impurities
  • Microbiological quality, where applicable
  • Bacterial endotoxins, where applicable
  • Stability testing

Storage

Store Nafarelin API in a tightly closed container under controlled pharmaceutical storage conditions, protected from excessive heat, moisture and direct light.

Because Nafarelin is a peptide API, final storage conditions should be based on validated stability data and the approved manufacturer's specification.

Pharmaceutical Research

Nafarelin is relevant to research involving:

  • GnRH receptor signaling
  • Luteinizing hormone regulation
  • Follicle-stimulating hormone regulation
  • Reproductive endocrinology
  • Endometriosis research
  • Sex-hormone regulation
  • Peptide drug development
  • Hormone-dependent disorders
  • Gonadotropin suppression
  • GnRH agonist research

Detailed Specifications

Parameter Specification
Appearance White crystalline powder or crystals
Identification IR & HPLC compliant
Assay (HPLC) 98.0% – 102.0%
Loss on Drying NMT 1.0%
Residue on Ignition NMT 0.5%
Individual Impurity NMT 0.5%
Total Impurities NMT 1.0%
Water Content NMT 1.0%
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