Mogamulizumab API Manufacturer in India

Swapnroop Pharma · WHO-GMP Certified · +91 87670 62101

Mogamulizumab API

Mogamulizumab is a humanized monoclonal antibody that selectively targets CC chemokine receptor 4 (CCR4). It is an anti-CCR4 therapeutic antibody used in oncology, particularly for the treatment of certain T-cell lymphomas.

Mogamulizumab is a complex biological drug substance rather than a conventional small-molecule API. It is an IgG1 kappa monoclonal antibody produced using recombinant DNA technology in mammalian cells.

Mogamulizumab API Manufacturer in India

Mogamulizumab is a specialized biological oncology API used in the development and manufacture of antibody-based pharmaceutical products. Quality control requires characterization of the antibody's identity, purity, potency, glycosylation profile, aggregates, charge variants and other critical quality attributes.

Chemical / Biological Information

CAS Number: 1262947-49-2
Molecular Formula: Not applicable as a conventional small molecule
Molecular Weight: Approximately 149 kDa
Molecule Type: Recombinant Humanized Monoclonal Antibody
Antibody Class: IgG1 Kappa
Target: CCR4 (CC Chemokine Receptor 4)
Drug Class: Monoclonal Antibody
Therapeutic Class: Antineoplastic / Immunotherapy
Therapeutic Area: Oncology / Hematology
Development Code: KW-0761
Brand Name: POTELIGEO

Because mogamulizumab is a glycoprotein produced biologically, its molecular mass can vary depending on glycosylation and other post-translational characteristics.

Mechanism of Action

Mogamulizumab binds specifically to CCR4, a chemokine receptor expressed on various T-cell populations, including malignant cells in certain T-cell lymphomas.

The antibody is engineered with an enhanced antibody-dependent cellular cytotoxicity (ADCC) profile. Following binding to CCR4-expressing cells, it can promote immune-mediated destruction of targeted cells through Fc-mediated effector mechanisms.

Pharmaceutical Applications

Mogamulizumab API is relevant to:

  • Oncology pharmaceutical development
  • CCR4-targeted cancer research
  • Cutaneous T-cell lymphoma research
  • Adult T-cell leukemia/lymphoma research
  • Monoclonal antibody development
  • Antibody-based cancer immunotherapy
  • Biopharmaceutical development
  • Analytical and quality-control testing

Oncology Applications

Mogamulizumab is particularly associated with CCR4-positive T-cell malignancies. Its targeted mechanism provides a research and therapeutic platform for investigating malignant T cells expressing CCR4.

It is used in the development of pharmaceutical products for certain patients with cutaneous T-cell lymphoma (CTCL) and adult T-cell leukemia/lymphoma (ATL), subject to applicable regulatory approvals and labeling.

Biological Characteristics

Mogamulizumab is a humanized IgG1 monoclonal antibody with a molecular mass of approximately 149 kDa. As a recombinant biological molecule, its quality is determined by multiple structural and functional attributes rather than only conventional small-molecule chemical purity.

Important characteristics include:

  • Antibody identity
  • Primary amino-acid sequence
  • Glycosylation profile
  • Aggregation level
  • Charge variants
  • Purity
  • Binding activity
  • Biological potency
  • Host-cell protein levels
  • Residual host-cell DNA
  • Bioburden and endotoxin

Manufacturing Process

A controlled mogamulizumab drug-substance manufacturing process may involve:

  1. Development and qualification of the recombinant cell line
  2. Cell-bank establishment
  3. Mammalian cell culture
  4. Controlled bioreactor production
  5. Harvest and clarification
  6. Protein A capture chromatography
  7. Viral inactivation
  8. Polishing chromatography
  9. Viral filtration
  10. Concentration and buffer exchange
  11. Sterile filtration
  12. Final quality-control testing
  13. Controlled storage and packaging

Quality Control Testing

Typical mogamulizumab quality testing may include:

  • Identity testing
  • Peptide mapping
  • Intact-mass analysis
  • Purity by chromatography
  • Size-exclusion chromatography
  • Aggregate determination
  • Charge-variant analysis
  • Glycan profiling
  • CCR4 binding assay
  • Biological potency assay
  • Host-cell protein testing
  • Residual host-cell DNA
  • Protein concentration
  • Endotoxin
  • Bioburden
  • Sterility, where applicable
  • Stability testing

Storage

Mogamulizumab drug substance should be stored under validated temperature-controlled conditions appropriate for the biological material. Protection from excessive temperature variation, light and physical stress is important. Final storage conditions should always follow the approved product specification and validated stability data.

Pharmaceutical Research

Mogamulizumab is relevant to research involving:

  • CCR4 biology
  • T-cell malignancies
  • Cutaneous T-cell lymphoma
  • Adult T-cell leukemia/lymphoma
  • Monoclonal antibody therapeutics
  • Antibody-dependent cellular cytotoxicity
  • Cancer immunotherapy
  • Targeted oncology
  • Recombinant antibody development
  • Biopharmaceutical analytics

Mogamulizumab API Summary

Mogamulizumab is a CCR4-directed humanized IgG1 monoclonal antibody with CAS Number 1262947-49-2 and an approximate molecular weight of 149 kDa. It is a biological oncology drug substance developed for targeted treatment of certain T-cell malignancies and is characterized through extensive structural, biochemical and biological quality testing.

Detailed Specifications

Parameter Specification
Appearance White crystalline powder or crystals
Identification IR & HPLC compliant
Assay (HPLC) 98.0% – 102.0%
Loss on Drying NMT 1.0%
Residue on Ignition NMT 0.5%
Heavy Metals NMT 10 ppm
pH (1% Solution) 2.0 – 4.0
Individual Impurity NMT 0.5%
Total Impurities NMT 1.0%
Water Content NMT 1.0%
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