Migalastat Hydrochloride API Manufacturer in India

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Migalastat Hydrochloride API

Migalastat Hydrochloride is a small-molecule pharmacological chaperone and hydrochloride salt of migalastat. It is used in the development and manufacture of pharmaceutical formulations for the treatment of Fabry disease in patients with amenable mutations in the α-galactosidase A gene.

Migalastat is an iminosugar that binds selectively and reversibly to certain mutant forms of α-galactosidase A (α-Gal A). This interaction can help stabilize the enzyme and promote its trafficking to lysosomes, where it can contribute to the breakdown of accumulated globotriaosylceramide and related substrates.

Migalastat Hydrochloride API Manufacturer in India

Migalastat Hydrochloride is a specialized rare-disease and metabolic-disorder pharmaceutical API used for oral pharmaceutical formulation development.

Important API quality attributes may include identity, assay, related substances, water content, residual solvents, elemental impurities, optical purity and other product-specific analytical parameters.

Chemical Information

CAS Number: 75172-81-5

Molecular Formula: C₆H₁₃NO₄·HCl

Molecular Weight: 199.63 g/mol

Chemical Name: (+)-(2R,3S,4R,5S)-2-(hydroxymethyl)piperidine-3,4,5-triol hydrochloride

Active Moiety: Migalastat

Drug Class: Pharmacological Chaperone

Therapeutic Class: Treatment of Fabry Disease

Therapeutic Area: Rare Diseases / Metabolic Disorders

Molecule Type: Small-Molecule Iminosugar

Mechanism of Action

Migalastat Hydrochloride acts as a pharmacological chaperone for α-galactosidase A.

Its pharmacological activity includes:

  • Selective binding to amenable mutant α-Gal A
  • Stabilization of certain mutant enzyme forms
  • Promotion of enzyme trafficking to lysosomes
  • Enhancement of intracellular α-Gal A activity
  • Facilitation of lysosomal substrate breakdown
  • Reduction of accumulated globotriaosylceramide and related substrates

Migalastat binds to the active site of certain mutant α-Gal A enzymes and stabilizes them, helping the enzyme reach the lysosome and retain activity.

Pharmaceutical Applications

Migalastat Hydrochloride API is used in pharmaceutical development for:

  • Fabry disease formulations
  • Pharmacological chaperone development
  • α-Galactosidase A research
  • Lysosomal storage disorder research
  • Rare-disease pharmaceutical development
  • Enzyme stabilization research
  • Metabolic disease research
  • Pharmaceutical formulation development

Fabry Disease Applications

Migalastat Hydrochloride is particularly relevant to Fabry disease, a lysosomal storage disorder associated with deficient or dysfunctional α-galactosidase A.

Migalastat is used for patients with Fabry disease who have amenable mutations, where the pharmacological chaperone mechanism can increase the functional activity of the patient's endogenous α-Gal A enzyme.

Physical Characteristics

Migalastat Hydrochloride is described as a white to almost white crystalline solid. It is freely soluble in aqueous media within the pH range of 1.2 to 7.5.

The crystalline form, water content, purity and other physicochemical characteristics should be controlled according to the applicable API specification.

Manufacturing Process

A controlled Migalastat Hydrochloride API manufacturing process may involve:

  1. Preparation of qualified starting materials
  2. Synthesis of the stereochemically defined migalastat intermediate
  3. Controlled stereochemical processing
  4. Purification
  5. Formation of the hydrochloride salt
  6. Crystallization
  7. Filtration
  8. Controlled drying
  9. Milling, where required
  10. Final analytical testing
  11. GMP-compliant packaging

The final process and controls should follow the approved manufacturing process and applicable pharmaceutical quality requirements.

Quality Control Testing

Typical Migalastat Hydrochloride API testing may include:

  • Identification by IR
  • HPLC identification
  • Assay
  • Related substances
  • Chiral purity / stereochemical purity
  • Water content
  • Residual solvents
  • Elemental impurities
  • Residue on ignition, where applicable
  • Optical rotation, where applicable
  • Crystalline-form characterization
  • Stability testing

Final numerical acceptance criteria should be taken from the current validated API specification or CoA rather than using generic limits.

Storage

Store Migalastat Hydrochloride API in a tightly closed container under controlled pharmaceutical storage conditions, protected from excessive moisture, heat and direct light.

For reference, the approved pharmaceutical product is stored at 20–25°C, with permitted excursions between 15–30°C; API storage should ultimately follow the validated stability data and approved API specification.

Pharmaceutical Research

Migalastat Hydrochloride is relevant to research involving:

  • Fabry disease
  • α-Galactosidase A
  • Pharmacological chaperones
  • Lysosomal storage disorders
  • Enzyme stabilization
  • Globotriaosylceramide metabolism
  • Rare-disease drug development
  • Metabolic disease research
  • Protein folding and trafficking
  • Pharmaceutical formulation development

Migalastat Hydrochloride API Summary

Migalastat Hydrochloride is a small-molecule pharmacological chaperone associated with the treatment of Fabry disease in patients with amenable α-galactosidase A mutations. It has CAS Number 75172-81-5, molecular formula C₆H₁₃NO₄·HCl, and molecular weight 199.63 g/mol.

Detailed Specifications

Parameter Specification
Appearance White crystalline powder or crystals
Identification IR & HPLC compliant
Assay (HPLC) 98.0% – 102.0%
Loss on Drying NMT 2.0%
Residue on Ignition NMT 0.5%
Individual Impurity NMT 0.5%
Total Impurities NMT 1.0%
Water Content NMT 1.0%
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