Lomustine API Manufacturer in India

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Lomustine API

Lomustine is an orally active alkylating antineoplastic agent belonging to the nitrosourea class. It is a lipophilic chemotherapy drug that can cross the blood-brain barrier, making it particularly relevant in the treatment of certain brain tumors and other cancers.

Lomustine acts by producing reactive alkylating species that damage DNA and interfere with DNA replication and cellular proliferation. It is also known by the development code CCNU.

Lomustine API Manufacturer in India

Lomustine is a specialized oncology pharmaceutical API used in the manufacture of oral anticancer formulations. Its production requires controlled synthesis, purification and stringent quality-control testing because nitrosourea compounds require careful handling and characterization.

Important quality attributes include identity, assay, related substances, water content, residue on ignition and residual solvents.

Chemical Information

CAS Number: 13010-47-4
Molecular Formula: C9H16ClN3O2
Molecular Weight: 233.69 g/mol
Synonym: CCNU
Chemical Name: 1-(2-Chloroethyl)-3-cyclohexyl-1-nitrosourea
Drug Class: Nitrosourea Alkylating Agent
Therapeutic Class: Antineoplastic Agent
Therapeutic Area: Oncology
Molecule Type: Synthetic Small Molecule

Mechanism of Action

Lomustine undergoes decomposition to form reactive metabolites capable of alkylating DNA and other cellular macromolecules.

DNA damage caused by these reactive intermediates interferes with:

  • DNA replication
  • DNA transcription
  • DNA repair
  • Cancer-cell proliferation

Its lipophilic nature allows Lomustine to cross the blood-brain barrier, contributing to its use in certain central nervous system malignancies.

Pharmaceutical Applications

Lomustine is used in oncology treatment regimens for certain:

  • Brain tumors
  • Gliomas
  • Glioblastoma
  • Hodgkin lymphoma
  • Other selected malignant diseases

Its clinical use depends on the approved indication, treatment protocol and applicable regulatory labeling.

Oncology Applications

Lomustine is particularly relevant to central nervous system oncology because of its ability to penetrate the blood-brain barrier.

It may be used alone or as part of combination chemotherapy regimens depending on the cancer type and treatment protocol.

Physical Characteristics

Lomustine is generally described as a yellow crystalline powder. It is practically insoluble in water and has greater solubility in certain organic solvents.

Manufacturing Process

A controlled Lomustine API manufacturing process may involve:

  1. Preparation of qualified starting materials
  2. Synthesis of the substituted urea intermediate
  3. Introduction of the cyclohexyl group
  4. Chlorination/substitution chemistry
  5. Nitrosation under controlled conditions
  6. Purification
  7. Crystallization
  8. Filtration
  9. Controlled drying
  10. Final analytical testing
  11. GMP-compliant packaging

Because Lomustine is a nitrosourea compound, manufacturing controls should include appropriate containment, impurity control and validated analytical procedures.

Quality Control Testing

Typical Lomustine API testing may include:

  • Identification by IR
  • HPLC identification
  • Assay by HPLC
  • Related substances
  • Nitrosourea-related impurities
  • Water content
  • Residue on ignition
  • Residual solvents
  • Elemental impurities
  • Melting point
  • Stability testing

Storage

Store Lomustine API in a tightly closed container under controlled pharmaceutical storage conditions, protected from heat, moisture and direct light. The final storage requirements should follow validated stability data and the applicable approved API specification.

Pharmaceutical Research

Lomustine is relevant to research involving:

  • Nitrosourea chemotherapy
  • DNA alkylation
  • DNA damage
  • Brain cancer
  • Glioblastoma
  • Glioma
  • Central nervous system tumors
  • Oncology drug development
  • Alkylating agents
  • Anticancer pharmaceutical formulations

Lomustine API Summary

Lomustine is a nitrosourea alkylating anticancer API with CAS Number 13010-47-4, molecular formula C9H16ClN3O2, and molecular weight 233.69 g/mol. Also known as CCNU, it produces DNA damage through alkylation and is notable for its ability to cross the blood-brain barrier.

Detailed Specifications

Parameter Specification
Appearance White to off-white crystalline powder
Identification Conforms by IR and HPLC
Assay (HPLC) 98.0% – 102.0%
Loss on Drying NMT 1.0%
Residue on Ignition NMT 0.5%
Heavy Metals NMT 20 ppm
Individual Impurity NMT 0.5%
Total Impurities NMT 2.0%
Water Content NMT 1.0%
Melting Point Approximately 90°C – 95°C
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