Lanadelumab API | Anti-Kallikrein Monoclonal Antibody API Manufacturer in India

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Lanadelumab API

Lanadelumab is a fully human monoclonal antibody that selectively targets and inhibits plasma kallikrein. It is a targeted biologic therapy used to reduce the frequency of hereditary angioedema (HAE) attacks.

By inhibiting plasma kallikrein activity, lanadelumab reduces the generation of bradykinin, a key mediator responsible for increased vascular permeability and swelling associated with hereditary angioedema.

Lanadelumab API Manufacturer in India

Lanadelumab is a specialized recombinant monoclonal antibody pharmaceutical ingredient requiring mammalian cell-culture expression, sophisticated purification and extensive biological characterization.

Critical quality attributes include identity, purity, aggregates, molecular integrity, charge variants, glycosylation profile, plasma-kallikrein binding/activity, biological potency, host-cell proteins, residual DNA, endotoxins and sterility.

Biological Information

CAS Number: 1306037-74-2
Molecular Formula: Not applicable as a conventional small molecule
Molecular Weight: Approximately 147 kDa
Drug Class: Plasma Kallikrein Inhibitor
Pharmacological Class: Anti-Plasma Kallikrein Monoclonal Antibody
Therapeutic Class: Biologic / Antiangioedema Agent
Therapeutic Area: Immunology / Rare Diseases

Mechanism of Action

Lanadelumab binds to plasma kallikrein and inhibits its proteolytic activity.

Plasma kallikrein participates in the kallikrein-kinin system and contributes to the production of bradykinin. Excess bradykinin is an important mediator of the swelling associated with HAE.

Lanadelumab therefore:

  • Inhibits plasma kallikrein
  • Reduces bradykinin generation
  • Decreases vascular permeability
  • Helps prevent swelling episodes
  • Reduces the frequency of hereditary angioedema attacks

Pharmaceutical Applications

Lanadelumab is primarily used for routine prevention of hereditary angioedema attacks in appropriate patients.

It is a preventive therapy, rather than a treatment intended to rapidly reverse an acute HAE attack.

Anti-Kallikrein Characteristics

Lanadelumab provides targeted inhibition of plasma kallikrein, distinguishing it from therapies that target other components of the HAE pathway.

Its mechanism is particularly relevant to bradykinin-mediated angioedema and the regulation of the kallikrein-kinin system.

Recombinant Manufacturing

Typical manufacturing of lanadelumab may involve:

  1. Recombinant mammalian cell-line development
  2. Cell-culture expansion
  3. Monoclonal antibody expression
  4. Harvest and clarification
  5. Capture chromatography
  6. Viral inactivation
  7. Polishing chromatography
  8. Viral filtration
  9. Ultrafiltration / diafiltration
  10. Final formulation
  11. Sterile filtration
  12. Aseptic filling

Quality and Analytical Testing

Lanadelumab quality evaluation may include:

  • Identity testing
  • Protein concentration
  • Purity
  • Aggregate analysis
  • Fragment analysis
  • Charge-variant profiling
  • Molecular-weight characterization
  • Peptide mapping
  • Glycosylation analysis
  • Plasma-kallikrein binding
  • Biological potency
  • Host-cell protein
  • Residual host-cell DNA
  • Residual Protein A
  • Bacterial endotoxins
  • Bioburden
  • Sterility
  • Subvisible particles

Storage

Lanadelumab drug substance and finished product should be stored according to approved product requirements and validated stability data. Appropriate refrigerated storage and protection from freezing, excessive heat and inappropriate light exposure are important for maintaining protein integrity.

Pharmaceutical Research and Development

Lanadelumab is relevant to research involving:

  • Plasma kallikrein
  • Kallikrein-kinin system
  • Bradykinin signaling
  • Hereditary angioedema
  • Rare disease therapeutics
  • Monoclonal antibodies
  • Recombinant protein manufacturing
  • Biological drug development
  • Vascular permeability
  • Anti-kallikrein therapies

Lanadelumab API Summary

Lanadelumab is a fully human monoclonal antibody targeting plasma kallikrein, with CAS Number 1306037-74-2 and an approximate molecular weight of 147 kDa. By inhibiting plasma kallikrein and reducing bradykinin generation, it is used for the prevention of hereditary angioedema attacks.

Detailed Specifications

Parameter Specification
Appearance White to off-white crystalline powder
Identification IR & HPLC compliant
Assay (HPLC) 98.0% – 102.0%
Loss on Drying NMT 1.0%
Residue on Ignition NMT 0.5%
Individual Impurity NMT 0.5%
Total Impurities ≤ 1.5%
Water Content NMT 1.0%
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