Ixekizumab API | Anti-IL-17A Monoclonal Antibody API Manufacturer in India

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Ixekizumab API

Ixekizumab is a humanized IgG4 monoclonal antibody that selectively targets interleukin-17A (IL-17A). It is an immunomodulatory biologic used in the treatment of several chronic inflammatory and immune-mediated diseases, particularly dermatological and rheumatologic conditions.

Ixekizumab binds to IL-17A and inhibits its interaction with the IL-17 receptor, thereby reducing inflammatory signaling involved in disease pathogenesis.

Ixekizumab API Manufacturer in India

Ixekizumab is a specialized recombinant monoclonal antibody pharmaceutical ingredient produced using mammalian cell-culture technology.

Quality evaluation requires characterization of biological and physicochemical attributes including identity, purity, aggregates, charge variants, glycosylation, molecular integrity, IL-17A binding activity, biological potency, host-cell proteins, residual DNA, endotoxins and sterility.

Biological Information

CAS Number: 1148119-45-0
Molecular Formula: Not applicable as a conventional small molecule
Molecular Weight: Approximately 146 kDa
Drug Class: Immunomodulatory Agent
Pharmacological Class: Anti-IL-17A Monoclonal Antibody
Therapeutic Class: Immunotherapy / Anti-inflammatory Biologic
Therapeutic Area: Dermatology / Rheumatology

Mechanism of Action

Ixekizumab selectively binds to IL-17A, including IL-17A homodimers and IL-17A/F heterodimers.

By neutralizing IL-17A, ixekizumab prevents IL-17A-mediated signaling through its receptor and reduces downstream inflammatory responses.

This can result in:

  • Reduced inflammatory cytokine signaling
  • Reduced recruitment and activation of inflammatory cells
  • Reduction of keratinocyte-mediated inflammation
  • Improvement in inflammatory skin lesions
  • Reduction of joint and enthesitis-related inflammation

Pharmaceutical Applications

Ixekizumab is used for selected immune-mediated inflammatory conditions, including:

  • Moderate-to-severe plaque psoriasis
  • Psoriatic arthritis
  • Ankylosing spondylitis
  • Non-radiographic axial spondyloarthritis
  • Other approved IL-17A-mediated inflammatory indications depending on jurisdiction

Specific indications depend on the applicable regulatory approval, patient age and product labeling.

Anti-IL-17A Characteristics

IL-17A is an important inflammatory cytokine involved in host defense and chronic inflammatory disease.

Ixekizumab provides targeted cytokine neutralization, making it relevant to diseases in which excessive IL-17 signaling contributes to inflammation.

Recombinant Manufacturing

Typical manufacturing of ixekizumab may involve:

  1. Recombinant cell-line development
  2. Mammalian cell culture
  3. Antibody expression
  4. Harvest and clarification
  5. Protein capture
  6. Chromatographic purification
  7. Viral inactivation
  8. Viral filtration
  9. Polishing
  10. Ultrafiltration / diafiltration
  11. Final formulation
  12. Sterile filtration
  13. Aseptic filling

Quality and Analytical Testing

Ixekizumab quality evaluation may include:

  • Identity testing
  • Protein concentration
  • Purity
  • Aggregate analysis
  • Fragment analysis
  • Charge-variant profiling
  • Molecular-weight characterization
  • Peptide mapping
  • Glycosylation analysis
  • IL-17A binding activity
  • Biological potency
  • Host-cell protein
  • Residual host-cell DNA
  • Residual Protein A
  • Bacterial endotoxins
  • Bioburden
  • Sterility
  • Subvisible particles

Storage

Ixekizumab drug substance and finished products should be stored according to validated stability data and approved product requirements. Appropriate refrigerated storage and protection from freezing, excessive heat and inappropriate light exposure are important for maintaining antibody integrity.

Pharmaceutical Research and Development

Ixekizumab is relevant to research involving:

  • IL-17A biology
  • Cytokine signaling
  • Monoclonal antibody therapeutics
  • Psoriasis
  • Psoriatic arthritis
  • Spondyloarthritis
  • Autoimmune and inflammatory diseases
  • Recombinant antibody production
  • Immunology
  • Biologic drug development

Ixekizumab API Summary

Ixekizumab is a humanized IgG4 anti-IL-17A monoclonal antibody with CAS Number 1148119-45-0 and an approximate molecular weight of 146 kDa. It selectively neutralizes IL-17A signaling and is used for selected chronic inflammatory and immune-mediated diseases.

Detailed Specifications

Parameter Specification
Appearance White crystalline powder
Identification IR & HPLC compliant
Assay (HPLC) 98.0% – 102.0%
Loss on Drying NMT 1.0%
Residue on Ignition NMT 0.5%
Individual Impurity NMT 0.5%
Total Impurities NMT 1.0%
Water Content NMT 1.0%
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