Itolizumab API | Anti-CD6 Monoclonal Antibody API Manufacturer in India

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Itolizumab API

Itolizumab is a humanized IgG1 monoclonal antibody that targets CD6, a cell-surface glycoprotein expressed primarily on T lymphocytes. By binding to CD6, it modulates T-cell activation and signaling and can reduce pro-inflammatory immune responses.

Itolizumab is an immunomodulatory biological therapeutic investigated and used in selected immune-mediated and inflammatory conditions.

Itolizumab API Manufacturer in India

Itolizumab is a specialized recombinant monoclonal antibody pharmaceutical ingredient produced using mammalian cell-culture technology.

Quality assessment requires comprehensive characterization of biological and physicochemical attributes, including identity, purity, aggregates, charge variants, glycosylation, molecular weight, CD6-binding activity, biological potency, host-cell proteins, residual DNA, endotoxins and sterility.

Biological Information

CAS Number: 113158-72-4
Molecular Formula: Not applicable as a single defined small-molecule formula
Molecular Weight: Approximately 150 kDa
Molecule Type: Humanized Recombinant Monoclonal Antibody
Drug Class: Immunomodulatory Agent
Pharmacological Class: Anti-CD6 Monoclonal Antibody
Therapeutic Class: Immunotherapy
Therapeutic Area: Immunology / Inflammatory Diseases

Mechanism of Action

Itolizumab binds to CD6, a receptor involved in T-cell activation, differentiation and migration.

CD6 engagement can influence signaling pathways involved in inflammatory immune responses. By targeting CD6, itolizumab can modulate T-cell activity and reduce the production or activity of inflammatory mediators.

Its immunomodulatory effects include:

  • Modulation of T-cell activation
  • Reduction of inflammatory signaling
  • Modulation of T-cell migration
  • Reduction of selected pro-inflammatory cytokines

Pharmaceutical Applications

Itolizumab has been developed for selected immune-mediated and inflammatory diseases, including:

  • Psoriasis
  • Rheumatoid arthritis
  • Other T-cell-mediated inflammatory disorders

Itolizumab has also been investigated in other inflammatory conditions and disease settings. Specific indications depend on the applicable regulatory approval and product formulation.

CD6 Targeting

CD6 is a co-receptor expressed on T cells and participates in interactions between T cells and antigen-presenting cells.

Targeting CD6 represents an approach to controlling pathological T-cell-mediated inflammation while modulating rather than completely suppressing immune function.

Recombinant Manufacturing

Manufacturing of Itolizumab generally involves:

  1. Recombinant antibody expression
  2. Mammalian cell culture
  3. Harvest and clarification
  4. Protein recovery
  5. Chromatographic purification
  6. Viral inactivation
  7. Viral filtration
  8. Concentration and buffer exchange
  9. Final polishing
  10. Sterile filtration
  11. Aseptic filling

Quality and Analytical Testing

Itolizumab quality evaluation may include:

  • Identity testing
  • Protein concentration
  • Purity
  • Size-exclusion chromatography
  • Aggregate analysis
  • Charge-variant analysis
  • Peptide mapping
  • Glycosylation characterization
  • Molecular-weight determination
  • CD6-binding activity
  • Biological potency
  • Host-cell protein testing
  • Residual host-cell DNA
  • Residual Protein A
  • Bacterial endotoxins
  • Bioburden
  • Sterility
  • Subvisible particles

Storage

Itolizumab drug substance and finished products should be stored according to validated stability data and approved product requirements. Controlled temperature conditions are essential for maintaining antibody integrity, purity and biological activity.

Pharmaceutical Research and Development

Itolizumab is relevant to research involving:

  • CD6 biology
  • T-cell signaling
  • Immunomodulation
  • Autoimmune diseases
  • Psoriasis
  • Rheumatoid arthritis
  • Inflammatory disorders
  • Monoclonal antibody therapeutics
  • Recombinant antibody manufacturing

Itolizumab API Summary

Itolizumab is a humanized IgG1 anti-CD6 monoclonal antibody with an approximate molecular weight of 150 kDa. It modulates T-cell activation and inflammatory signaling through interaction with CD6 and has been developed for selected immune-mediated and inflammatory diseases.

Detailed Specifications

Parameter Specification
Appearance White to off-white crystalline powder
Identification IR & HPLC compliant
Assay (HPLC) 98.0% – 102.0%
Loss on Drying NMT 1.0%
Residue on Ignition NMT 0.5%
Individual Impurity NMT 0.5%
Total Impurities NMT 1.0%
Water Content NMT 1.0%
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