Isepamicin API | Aminoglycoside Antibiotic API Manufacturer in India

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Isepamicin API

Isepamicin is a semisynthetic aminoglycoside antibiotic structurally related to gentamicin B. It exhibits antibacterial activity against a range of Gram-negative bacteria and was developed to provide improved resistance to certain aminoglycoside-inactivating enzymes.

Isepamicin acts primarily by binding to the 30S bacterial ribosomal subunit, interfering with bacterial protein synthesis and producing a bactericidal effect.

Isepamicin API Manufacturer in India

Isepamicin is a specialized aminoglycoside antibacterial API manufactured through controlled chemical and/or semisynthetic processes followed by purification and analytical characterization.

Important quality attributes include identity, assay, related substances, water content, sulfate content where applicable, residual solvents, elemental impurities, microbial quality and stability.

Chemical Information

CAS Number: 58152-03-7
Molecular Formula: C22H43N5O12
Molecular Weight: 585.60 g/mol
Drug Class: Aminoglycoside Antibiotic
Pharmacological Class: Aminoglycoside Antibacterial
Therapeutic Class: Antibacterial Agent
Therapeutic Area: Infectious Diseases

Mechanism of Action

Isepamicin binds to the 30S subunit of bacterial ribosomes. This interferes with accurate translation of messenger RNA and results in the production of abnormal proteins.

Its antibacterial activity ultimately leads to:

  • Inhibition of bacterial protein synthesis
  • Misreading of genetic information
  • Disruption of bacterial cell function
  • Bacterial cell death

Isepamicin is therefore considered a bactericidal aminoglycoside.

Antibacterial Activity

Isepamicin has activity primarily against susceptible aerobic Gram-negative organisms. Its activity against particular bacterial strains depends on susceptibility patterns and resistance mechanisms.

A key characteristic of isepamicin is its relative stability against certain aminoglycoside-modifying enzymes, although resistance can still occur through other mechanisms.

Pharmaceutical Applications

Isepamicin has been investigated or used in selected settings for treatment of serious bacterial infections, particularly infections caused by susceptible Gram-negative organisms.

Potential clinical applications include:

  • Serious Gram-negative infections
  • Complicated urinary tract infections
  • Hospital-acquired infections
  • Resistant bacterial infections
  • Severe systemic bacterial infections

Use depends on local regulatory approval, antimicrobial susceptibility and applicable clinical guidelines.

Aminoglycoside Characteristics

Like other aminoglycosides, isepamicin demonstrates:

  • Concentration-dependent antibacterial activity
  • Post-antibiotic effect
  • Predominantly renal elimination
  • Activity against susceptible aerobic Gram-negative bacteria

Manufacturing Process

Typical production may involve:

  1. Preparation of the aminoglycoside intermediate
  2. Semisynthetic modification
  3. Reaction and controlled processing
  4. Purification
  5. Crystallization or isolation
  6. Drying
  7. Milling where required
  8. Final analytical testing
  9. Packaging under controlled conditions

Quality and Analytical Testing

Isepamicin API quality evaluation may include:

  • Identification
  • Assay
  • Related substances
  • Aminoglycoside impurities
  • Water content
  • Sulfate/ counter-ion determination where applicable
  • Residual solvents
  • Elemental impurities
  • Specific optical rotation where applicable
  • pH
  • Microbial limits
  • Endotoxins where applicable
  • Stability testing

Storage

Isepamicin should be stored in a tightly closed container under controlled pharmaceutical storage conditions, protected from excessive moisture, heat and direct light. Final storage requirements should be established from validated stability studies.

Pharmaceutical Research and Development

Isepamicin is relevant to research involving:

  • Aminoglycoside antibiotics
  • Antimicrobial resistance
  • Gram-negative bacterial infections
  • Ribosomal protein synthesis
  • Aminoglycoside-modifying enzymes
  • Antibiotic susceptibility
  • Semisynthetic antibacterial development

Isepamicin API Summary

Isepamicin is a semisynthetic aminoglycoside antibiotic with CAS Number 58152-03-7, molecular formula C22H43N5O12, and molecular weight 585.60 g/mol. It acts primarily by binding to the bacterial 30S ribosomal subunit and inhibiting accurate protein synthesis.

Detailed Specifications

Parameter Specification
Appearance White to off-white crystalline powder
Identification IR & HPLC compliant
Assay (HPLC) 98.0% – 102.0%
Loss on Drying NMT 1.0%
Residue on Ignition NMT 0.2%
pH (1% Solution) 2.0 – 4.0
Individual Impurity NMT 0.5%
Total Impurities NMT 1.0%
Water Content ≤5.0%
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