Isatuximab API | Anti-CD38 Monoclonal Antibody API Manufacturer in India

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Isatuximab API

Isatuximab is a chimeric IgG1 monoclonal antibody that targets CD38, a cell-surface glycoprotein highly expressed on malignant plasma cells. It is an important anti-CD38 monoclonal antibody used in multiple myeloma therapy.

By binding to CD38, isatuximab can directly and indirectly promote the destruction of CD38-expressing malignant cells through multiple immune-mediated mechanisms.

Isatuximab API Manufacturer in India

Isatuximab is a specialized recombinant monoclonal antibody pharmaceutical ingredient manufactured using mammalian cell-culture technology.

Its quality evaluation requires extensive characterization of biological and physicochemical attributes, including identity, purity, aggregation, charge variants, glycosylation, molecular weight, CD38-binding activity, biological potency, host-cell proteins, residual DNA, endotoxins and sterility.

Biological Information

CAS Number: 946635-39-4
Molecular Formula: C6426H9924N1708O2012S42
Molecular Weight: Approximately 148 kDa
Molecule Type: Recombinant Chimeric Monoclonal Antibody
Drug Class: Antineoplastic Agent
Pharmacological Class: Anti-CD38 Monoclonal Antibody
Therapeutic Class: Antineoplastic / Immunotherapy
Therapeutic Area: Oncology / Hematology

Mechanism of Action

Isatuximab binds to CD38, a transmembrane glycoprotein expressed at high levels on multiple myeloma cells.

CD38 binding can result in several antitumor mechanisms, including:

  • Antibody-dependent cellular cytotoxicity (ADCC)
  • Antibody-dependent cellular phagocytosis (ADCP)
  • Complement-dependent cytotoxicity (CDC)
  • Direct induction of apoptosis
  • Modulation of CD38 enzymatic activity
  • Immune-system activation against malignant plasma cells

Through these mechanisms, isatuximab contributes to the elimination of malignant plasma cells.

Pharmaceutical Applications

Isatuximab is used in combination regimens for the treatment of multiple myeloma in appropriate patients.

It has been used in combination with other antimyeloma agents, including:

  • Pomalidomide and dexamethasone
  • Carfilzomib and dexamethasone

The specific indication, treatment line and combination depend on the applicable regulatory approval.

CD38 Targeting

CD38 is expressed on plasma cells and plays roles in NAD metabolism, calcium signaling and immune-cell biology. Its high expression on malignant plasma cells makes it an important therapeutic target in multiple myeloma.

Isatuximab selectively recognizes a specific epitope on CD38 and produces antitumor activity through both immune-mediated and direct mechanisms.

Recombinant Manufacturing

Manufacturing of Isatuximab generally involves:

  1. Recombinant antibody expression
  2. Mammalian cell culture
  3. Harvest and clarification
  4. Protein recovery
  5. Chromatographic purification
  6. Viral inactivation
  7. Viral filtration
  8. Concentration and buffer exchange
  9. Final polishing
  10. Sterile filtration
  11. Aseptic filling

Quality and Analytical Testing

Isatuximab quality evaluation may include:

  • Identity testing
  • Protein concentration
  • Purity
  • Size-exclusion chromatography
  • Aggregate analysis
  • Charge-variant analysis
  • Peptide mapping
  • Glycosylation characterization
  • Molecular-weight determination
  • CD38-binding activity
  • Biological potency
  • Host-cell protein testing
  • Residual host-cell DNA
  • Residual Protein A
  • Bacterial endotoxins
  • Bioburden
  • Sterility
  • Subvisible particles

Storage

Isatuximab drug substance and finished products should be stored according to validated stability data and approved product requirements. Appropriate temperature control is essential to maintain antibody integrity, purity and biological activity.

Pharmaceutical Research and Development

Isatuximab is relevant to research involving:

  • CD38 biology
  • Multiple myeloma
  • Plasma-cell malignancies
  • Monoclonal antibody therapeutics
  • Antibody-dependent cytotoxicity
  • Cancer immunotherapy
  • Combination antimyeloma therapy
  • Recombinant antibody manufacturing

Isatuximab API Summary

Isatuximab is a chimeric IgG1 monoclonal antibody targeting CD38, with CAS Number 946635-39-4 and an approximate molecular weight of 148 kDa. It promotes the elimination of CD38-expressing malignant plasma cells through several immune-mediated and direct mechanisms and is used in combination therapy for multiple myeloma.

Detailed Specifications

Parameter Specification
Appearance White to off-white crystalline powder
Identification IR & HPLC compliant
Assay (HPLC) 98.0% – 102.0%
Loss on Drying NMT 1.0%
Residue on Ignition NMT 0.5%
Heavy Metals NMT 20 ppm
pH (1% Solution) 4.0 – 7.0
Individual Impurity NMT 0.5%
Total Impurities NMT 1.0%
Water Content NMT 1.0%
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